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Excess mitochondrial ROS production, causes cellular oxidative stress, sustains mitochondrial dysfunction and ROS production and perpetuates inflammation (Chang et al., 2021)
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As both H 2 O 2 and BCNU exposure led to excessive cellular GSSG accumulation, their impact on intracellular protein-GSSG adduct formation (protein S -glutathionylation) was subsequently examined in HepG2 cells by western blot analysis using a monoclonal GSSG antibody that specifically binds to GSSG-modified proteins
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