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It has been examined in laboratory and clinical research models related to metabolic regulation , energy balance , and feeding behavior

Cells were washed in PBS, resuspended in Thiazole Orange (BD Retic-Count, BD Bioscience) containing 4 M Hoechst 33342 (Invitrogen) for parasite RNA and DNA detection respectively ( 2 , 5 mM glucose, 32 mM HEPES pH7.4, 5 mM KCl, 1 mM MgSO4, 125 mM NaCl), resuspended in 0.3 M of intracellular calcium dye x-Rhod-1 AM (Invitrogen) and incubated in the dark at 37C for 30 min

Sterile Technique is to be maintained, aseptic handling is to be done to avoid contamination

however, as the disease progresses these can often be seen with the naked eye, particularly when the iris is lightly pigmented and there is severe copper overload[13]

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