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Methods In vitro experiments Biochemical binding assay Biochemical binding of TLC-2716 and TLC-6665 to LXR or LXR was determined by time-resolved fluorescence resonance energy transfer-based biochemical assays evaluating the concentration-dependent displacement of a N-terminally biotinylated coactivator NCOA3 to recombinant glutathione S -transferase (GST)-tagged LXR or LXR ligand-binding domain (LBD)