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coli containing plasmid pANT35 or pANT62 was inoculated from an overnight culture at a ratio of 1:100 in a 2 l baffled Erlenmeyer flask filled with 400 ml Zym-5052 medium 51 containing 50 g ml 1 kanamycin sulfate per flask, incubated at 20 C, 140 rpm, 5 cm shaking diameter for 24 h, and the final OD 600 was determined using a CO8000 cell density meter (WPA biowave)

Plate reader data were manually checked before analysis for technically invalid replicates, e.g

Why Antioxidants Matter in the First Place (and Why Glutathione is the Master) The antioxidant story all starts with rogue molecules called free radicals -- unstable molecules that can cause general mayhem in the cells

The protein molecular masses of the homologous proteins and the isoelectric points (pI) were predicted by using an ExPASy Compute pI/Mw tool ( ClustalX version 1.81 was used to perform the multiple sequence alignment

They sound similar, but are actually quite different