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Once the xenograft tumors for the study of 2DG or BPTES treatment were established (when the tumor volume was ~50100 mm 3 ), the mice were randomly divided into six groups ( n = 5 mice per group) and treated with saline buffer (10% DMSO), 2DG (500 mg/kg/day) or BPTES (12.5 mg/kg/day), each with or without TRAIL (5 g/kg/day), via intraperitoneal injection three times a week for 33 days

Storage and stability: Amber glass, refrigerated supply chain, and a slightly acidic pH (5 to 7) preserve the complex

Diabetes Care 45 (12), 30753090

Data availability All data analyzed in this publication were publicly available, including the human lung MSI and spotted mouse liver from METASPACE ( and the murine urinary bladder from the Proteomics Identifications Database (PXD001283) and the 10X Visium and MALDI (FMP-10 and DHB matrices) Parkinson mouse brain data ( The IDH -mutant tumor data are available from the BioImage Archive ( All data objects used for vignettes can also be found on the SpaMTP Zenodo community page ( Databases used for metabolite annotation include HMDB ( released November 2, 2021), ChEBI ( released September 1, 2023), LIPIDMAPS ( released September 15, 2023) and GNPS ( released April 21, 2025)

Mild temporary soreness at the injection site is possible