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The realistic expectation is hydration improvement visible within 48 hours, surface texture refinement by week 4, fine-line softening from week 8, and cumulative collagen-driven firmness from month 3 onward, sustained through bi-weekly sessions over 4 to 6 months

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37 In terms of antimicrobial activity, berberine demonstrated strong effects against dysbiotic bacteria in IBS models, reducing the abundance of Escherichia/Shigella and Proteobacteria while enriching beneficial taxa such as Lactobacillus and Ruminococcus

Clinical, Cosmetic and Investigational Dermatology, 267

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