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The complex was then concentrated using an Amicon Ultra Centrifugal Filter (MWCO, 100 kDa) and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column (GE Healthcare) that was pre-equilibrated with 20mM HEPES pH 7.4, 100mM NaCl, 2mM MgCl 2 , 1M peptide, 0.01% (w/v) LMNG and 0.0006% (w/v) CHS to separate complex from contaminants
El GLP-1 es una hormona intestinal que estimula la insulina, suprime el glucagn y retrasa el vaciamiento gstrico de manera glucosa-dependiente
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Additionally, studies in patients with T2DM are now needed to explore potential clinical benefits [197]
It helps maintain a strong heart, immune function, and eye and lung health
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