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Endothelial cell studies report increased proliferation, migration, and tube formation in culture systems that measure angiogenic signaling behavior

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OPCs were cultured on poly-l-lysine/laminin-coated plastic dishes in DMEM-F12 (Invitrogen) medium that was supplemented with 2.5 g/l NaHCO 3 , 1% N 2 supplement, 2% B27 supplement, 1% penicillin or streptomycin, 0.01% BSA, 5 g/ml insulin, 40 ng/ml FGF2 (Millipore), and 20 ng/ml PDGFaa, and approximately 15,000 cells/cm 2 were seeded on a PDL/laminin-coated 10 cm tissue culture plate