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In the study of the combined effects of paeoniflorin (PF), the main metabolite of Radix Paeoniae Alba, and glycyrrhizin (GLR), the main metabolite of Radix Glycyrrhizae, on the isolated sciatic nerve-gastrocnemius muscle preparation of frogs or the isolated in-situ phrenic nerve-diaphragm muscle preparation of mice, it was found that the combined use of PF and GLR can block the twitch caused by indirect stimulation
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