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Lovis, Genve (Sciences de linformation)
Isolated hepatocytes were plated on collagen-coated 6-well plates (4 10 4 cells per cm 2 ) in DMEM (Gibco #12800-017, containing 1 mM pyruvate, 25 mM glucose, and 4 mM glutamine) supplemented with 10 % (vol/vol) fetal bovine serum (FBS), 100 units/ml penicillin, 100 g/ml streptomycin, 1 M dexamethasone, 0.1 M insulin, and an additional 1 mM sodium pyruvate and incubated at 37 C in an atmosphere of 5 % CO 2
Immunity 50 , 520532.e3 (2019)
doi:10.1016/j.lfs.2019.117139 Park JR, Lee H, Kim SI, Yang SR
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