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Cells were imaged in fresh FluoroBrite DMEM supplemented with 4 mM glutamine, 1 mM sodium pyruvate, 10% FBS and penicillin (100 U ml 1 )/streptomycin (100 g ml 1 )/fungizone (0.25 g ml 1 )

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then, samples were processed using serial grades of ethanol and cleared in xylene followed by infiltration and embedding in a Paraplast tissue-embedding media

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The GLP-1(9-36)GLP-1RG s complex formation was initiated by the addition of 100 M GLP-1(9-36), 10 g/mL nanobody 35 (Nb35) and 25 mU/mL apyrase (New England Bio-Labs)