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The consequences are significant: accelerated muscle loss, a soft flabby appearance despite weight loss, reduced metabolic rate, weakened immune function, hair loss, and poor recovery from exercise

Management of endocrine diseasE: Are all GLP-1 agonist equal in the treatment of type 2 diabetes

DSS-induced colitis in mice elicited pronounced symptoms, including reduced locomotor activity, decreased consumption of food and water, dull and lifeless fur, signs of depression-like behavior, huddling behavior, perianal swelling and congestion, diarrhea, bloody stools, and substantial weight loss

Journal of Diabetes

Repeated freeze-thaw cycling of a reconstituted preparation promotes aggregation, so aliquoting once and thawing each aliquot only once is standard practice