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To assess the importance of lipidation modification to Cagri, pharmacological comparison of Cagri and its non-lipidated backbone (CagriBB) was undertaken using a cAMP response element (CRE)-reporter assay

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The key is duration-appropriate exposure: colder temperatures require shorter times

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In 3-AR knockout mice, however, no effect was observed with AOD9604 confirming the dependency of its chronic lipolytic action on 3-AR expression, while also suggesting an additional acute mechanism that may operate through a separate pathway