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Concentration Calculation Reference The formula for reconstitution concentration is straightforward: Concentration (mg/mL) = Peptide Mass (mg) / Volume of Solvent Added (mL) Or rearranged to find the volume needed: Volume of Solvent (mL) = Peptide Mass (mg) / Target Concentration (mg/mL) Example Calculations For very low-concentration preparations (mcg/mL range), prepare a higher-concentration stock first and dilute with additional solvent

After 12 h of incubation cells were washed by PBS, the cells adhering to the lower membrane of the inserts was counted after staining with 0.1% crystal violet

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Many researchers use a simple system: week 1 left abdomen, week 2 right abdomen, week 3 left thigh, week 4 right thigh