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To understand the effect of mutation on allosteric transmission, transmission of allosteric intensity was applied with RTA and changes in rigid clusters using FIRST (compared with wild type with mutated structures using FIRST at hydrogen bond energy strength 1.0 kcal/mol), we carried out same process (as described above) on all mutated structures (Gly376Asp, Ser441Leu and Arg217Cys)

Confirmed at 28 days A follow-up 28-day study (Babula et al., 2024) extended the finding: 5-Amino-1MQ dose-dependently limited body weight and fat mass gain without changing food intake, and it improved liver markers in obese mice