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The assay was conducted in the wells of 96-well black plastic microplates, in which 20 L of enzyme solution and 60 L of bile salt were mixed together, and then 20 L of substrate was added, with mixing, at the start of the reaction, which proceeded at room temperature for up to 30 min, with measurement at various intermediate time points

This prolongs and amplifies the physiologic effects of endogenous GLP-1

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Endothelial therapy can be achieved with primary endothelial therapy for prevention of healthy endothelial function by controlling cardiovascular risk factors and secondary endothelial therapy for to improve dysfunctional endothelial homeostasis by treating underlying cardiovascular risk factors and cardiovascular disease