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One cause of B12 deficiency includes pernicious anemia, a condition that results in a reduction of red blood cells when the intestines cannot absorb enough vitamin B12

This mechanism naturally limits absorption when B12 is consumed in large quantities, with excess amounts typically eliminated through the kidneys without causing harm

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Furthermore, DAT can also be regulated through direct protein-protein interactions with intracellular proteins such as -synuclein [21], synaptogyrin-3 [22], etc