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Labelled cells were then detached by trypsinization, pelleted, washed once with PBS, resuspended in 300 l ice-cold PBS and fixed by adding 700 l ice-cold absolute ethanol while gently vortexing

The studys primary outcome focused on major adverse CV events, and with a median follow-up of 47.5 months, those treated with oral semaglutide had a significant 14% reduction in a composite of the CV outcomes compared with those treated with placebo ( P = .006)

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