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HSV-1-Induced H2AX Accumulation Requires Virus Binding and Replication in Host Cells To check whether the accumulation of neuronal DNA damage is related to specific steps of virus life-cycle, we analyzed the formation of H2AX foci in neurons infected either with heat- or UV-inactivated viruses (i.e., unable to enter into the host cells or to replicate within its nucleus, respectively) or in the presence of PAA, a specific inhibitor of viral polymerase and thus of viral DNA synthesis