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The reaction mixture (1 mL) contained 50 mM potassium phosphate buffer (pH 7.0), 0.5 mM ascorbic acid, 0.1 mM HO, and a suitable aliquot (typically 50 L) of the enzyme extract

Energy Expenditure and Thermogenesis GLP3s glucagon receptor activation specifically contributes to increased energy expenditure through multiple pathways[6]: Enhanced hepatic fatty acid oxidation and lipolysis Increased thermogenic activity in brown adipose tissue Upregulation of uncoupling protein expression Potential FGF21-mediated metabolic effects This thermogenic component distinguishes the blend from pure GLP-1 or amylin-based approaches, addressing both energy intake (via satiety) and energy expenditure simultaneously

p values less than 0.05 were considered to denote statistical significance

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, BMD, bone mineral content, trabecular bone volume, alkaline phosphatase, and osteocalcin) and increased resorptive parameters ( e.g