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For quantitative TMAO analysis, plasma specimens were diluted 1:10 in acetonitrile containing a spiked isotopic standard, TMAO-D9 (Cambridge isotope DLM-4779-1)

This work was supported by grants from the Fondo Concursable de Investigacin 570609, Departamento de Tecnologa Mdica, Universidad de Chile (PC), ANID scholarship 21253303 (AP-H), ANID/FONDECYT 3240492 (MT), ANID/FONDECYT Grants 11251042 (ES-P), 11221017 (YH-F) and ANID/Basal FB210024 (YH-F)

Sterile water is the alternative diluent where benzyl alcohol is incompatible with the downstream assay

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Tirzepatide is a clinician-guided medication that supports appetite regulation and metabolic balance