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Further microscopy experiments performed to visualise GLP-1R engagement with EGFP-VAPB in response to exendin-4 stimulation include confocal imaging of serial optical sections covering the entire cell thickness after SNAP/FLAG-hGLP-1R surface labelling with a membrane impermeable SNAP-tag probe followed by exendin-4 stimulation, represented as a maximum intensity projection (MIP) in Fig

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