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Reverse-calculate concentration after reconstitution to verify accuracy before drawing the first dose

Enzyme expression was induced by adding 100 M isopropyl -d-1-thiogalactopyranoside, and the cultures were incubated for another 20.5 h at 20 C, 300 rpm, 5 cm shaking diameter

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However, for multi-dose research vials, bacteriostatic water (which contains 0.9% benzyl alcohol) is required to prevent microbial contamination after the first needle puncture