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After treatment, cells were incubated with C11-Bodipy for 30 minutes at 37C

Problem: Calculated concentration does not match expected bioassay response

Regular blood monitoring, which is already standard practice for methotrexate users, helps identify any emerging issues with liver function, kidney function, or blood counts that might be influenced by the combination of medications or by inadequate nutrition

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But others are gambling on a do-it-yourself strategy to ease off the drugs and stay slim by stretching out doses, taking the medication intermittently or stopping and starting again only if needed