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From day 8 to day 14, cells were cultured under normoxia in RPMI 1640 medium (Invitrogen) supplemented with 2 mM Gluta-MAX, 1 B-27 supplement (Invitrogen) and 50 g ml 1 AA

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Additional studies have demonstrated that combining GIP and GLP-1 receptor agonists is more effective at reducing body weight than using either individually