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Clinical samples Twenty subjects were recruited for this study, including 10 non-smokers without COPD, 10 smokers with COPD
Growth media was replaced with stimulation buffer [phenol-free DMEM containing 0.1% (w/v) bovine serum albumin (BSA) and 1 mM 3-isobutyl-1-methylxanthine] and incubated for 1 h at 37 C in 5% CO 2
Nature, 496 (7443), 101105
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