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Briefly, HaCaT keratinocytes were seeded at a density of 10,000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

Sequencing data from pooled lanes were demultiplexed, and after a default quality-filtering step (using FastQC_v.0.10.1), they were recorded in FastQ files representing raw data

Getting this step right protects both your results and the integrity of the compound, since sloppy handling degrades the peptide and wastes money

Neurobion products are combinations of B vitamins

We want to bridge that gap