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Cell viability assay (CCK-8) Stock preparations of OSR (10, 20, and 40 mM) were generated in DMSO, and recombinant human IL1 was adjusted to a final concentration of 10 ng/mL

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Such phosphorylation leads to the blockade of the tyrosine residues in IRSs, to which the insulin and IGF-1 receptors phosphorylate to activate IRSs and consequently commence the signalling cascade [185, 192], as comprehensively discussed above

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RNA isolation and quantitative real-time PCR assay HBE cells were seeded in 6-well plates at a density of about 1 10 6 cells per well, and exposed to 50, 100, 250, or 500 g/ml Al 2 O 3 NPs or control medium for 24 h, then cells were trypsinized and collected