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doi: 10.1016/j.clinbiochem.2023.02.008

Luo J, Li T, Yuan J

Kwong, M., Y.W

If solution storage is necessary, researchers commonly use sterile buffers around pH 56, aliquot to reduce freezethaw exposure, and store refrigerated shortterm (often referenced as up to ~30 days at ~4C (39F) as general guidance), freezing when not in use depending on the protocol

Generally, B12 shots are designed to provide a sustained boost in B12 levels, but the exact timeframe can depend on factors such as your metabolism, lifestyle, and any underlying health conditions