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Candida albicans is an obligate sugar-feeder: without dietary glucose and fructose, its proliferation is dramatically curtailed, its hyphal transition is suppressed, and antifungal agents are exponentially more effective
Repeated freeze-thaw cycles should be avoided, as they can degrade the peptide and reduce potency
In fact, people with vitamin B12 levels on the lower side of normal performed worse on memory tests than those with high-normal levels in a study in The American Journal of Clinical Nutrition (5)
After the first incubation, 50 L of the suspension was transferred into fresh SM liquid medium for the second round of enrichment