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UK regulators recommend stopping the medication at least two months before trying to conceive, because semaglutide has a long half-life of around one week and stays in the body for several weeks after the last dose

Peaks were annotated with the use of the Fiehn library (Agilent G1676AA Fiehn GC/MS Metabolomics RTL Library, User Guide, Agilent Technologies, Metabolic features with low reproducibility or linearity were removed from the dataset, resulting in 102 annotated metabolic features

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Quantitative data were presented as mean standard deviation (SD), and a two-sided T -test was used to compare the means between the intervention and control groups