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When introducing the diluent to the vial, aim the needle against the inner wall of the glass, allowing the liquid to slide down slowly rather than injecting it directly into the powder
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In the liver, the 25-hydroxylase enzymes (CYP2R1 and CYP27A1) convert inactive cholecalciferol to 25(OH)VD 3 (calcidiol) by carbon 25 hydroxylation 14
Similar findings were observed using 15 N 13 C-cystine and by performing high-resolution proteomics (Fig
10 , e020734 (2021)
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