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Onephoton fluorescence setup One-photon fluorescence measurements were performed with Typhoon FLA 9500 biomolecular imager using a 473 nm (blue LD laserfor NCs detection) or a 685 nm laser (Red LD laserfor AlexaFluor700 detection) for excitation and a BPFR700 (R715) filter to collect the emitted fluorescence in the wavelength range from 713 nm to 726 nm

We generally do not recommend freezing reconstituted peptides

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Bacteriostatic water is the best general-purpose solvent because its 0.9% benzyl alcohol preservative protects multi-use vials for weeks