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In-vitro fibroblast studies indicate that GHK-Cu upregulates transcription of collagen and elastin genes and supplies copper as a cofactor for lysyl oxidase, the enzyme that cross-links these matrix proteins

Treatment Protocol Research Limitations Moderate sample size (n=45)

At the 20-min time point, Visualization of fibrils was carried out for morphological characterization using fluorescence microscopy and transmission electron microscopy (TEM), following the protocols outlined earlier