Recordings were made at room temperature in artificial cerebrospinal fluid (aCSF) (pH 7.3, osmolarity 305 +/- 5 mOsm/kg) perfused at 2 mL/min containing 150 mM NaCl, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 10 mM glucose, 3 mM potassium chloride, 2 mM calcium chloride, and 1.3 mM magnesium chloride
As a future direction, we propose testing whether, in the absence of Trim37 , PGCs progressively lose intrinsic germline determinants and fail to stabilize a coherent somatic identity because they do not receive appropriate lineage-specifying cues, ultimately resulting in elimination through cell death
These mechanisms operate in different cellular compartments and do not directly compete for metabolism or clearance pathways
Additionally, the WD proteins play a significant role in determining anthocyanin biosynthesis
Lauren B