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For drug treatments and qPCR analyses, hiPSC-CM were seeded on gelatin- or Matrigel-coated 12-well plates at 500,000 cells/well in medium containing RPMI 1640, B-27 supplement, 10 M ROCK inhibitor and 10% fetal bovine serum

The oxidative stress also contributes to atherosclerosis, representing a possible link between NAFLD and metabolic syndrome (37)

Structured trials speed discovery: change one variable for 3 to 5 days and log meals, dosing, and symptoms, since a 4-week symptom-log analysis showed isolating variables rapidly uncovers repeatable triggers

Diabetes & Metabolic Syndrome, 11 Suppl 2, S631S635