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O.TonelliM.MarkleyJ
Lu Y, Li X, Geng D, Mei N, Wu PY, Huang CC, et al
Expi293F cells were grown in Union-293 medium and used for protein expression, with 1 mg of total DNA plasmid and 3 mg of PEI (Biohub, 78PEI40000-1g) per 1 liter of cells at a density of 1.5 2.0 10 6 cells/ml
However, selective knockdown of GLP-1R in the DVC only partially perturbs normal energy balance, whereas GLP-1R knockdown in the hypothalamus does not result in any energy balance impairment 60
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