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The site you choose depends on three things: How quickly the medication is absorbed into your bloodstream How comfortable the injection is The state of your skin over time Unlike oral medications that are absorbed through the gut, injections rely on a consistent layer of fat tissue to ensure steady, predictable drug absorption

Methylfolate is required in the activation of tetrahydrobiopterin (i.e., BH4), which is a necessary cofactor for both tryptophan hydroxylase and tyrosine hydroxylase, enzymes required in the synthesis of serotonin and norepinephrine/dopamine, respectively (Figure 1)

A single colony was inoculated into 100 ml of LB medium containing the appropriate antibiotics and incubated at 37 C overnight