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For the reverse transfection on a Freedom EVO 200 workstation (Tecan), 5 l of 250 nM siRNA per well, diluted in Opti-MEM (Thermo Fisher Scientific), were placed into a 384-well PhenoPlate (PerkinElmer) followed by the addition of 0.02 l RNAiMAX (Thermo Fisher Scientific) in 5 l Opti-MEM using a BioTek EL406 Washer Dispenser (Agilent)
Subtle B12 deficiency may be present even if the blood tests are in the normal range
Serum analysis Mice were anesthetized with isoflurane, after which blood was collected via the retro-orbital venous sinus into BD microtainer tubes (BD #365967)
Although it can be quite uncomfortable, the reaction is usually temporary and indicates that the treatment is working
What Is GHK-Cu and How Does It Work
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