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Third, we chose to use overall survival as the primary endpoint, which is a hard outcome, providing a comprehensive assessment of the treatment effect in a retrospective study, although the proportion of patients becoming non-self-sufficient and ALSFRS-R total score, used as secondary outcomes, are validated endpoints for trials in ALS

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These findings demonstrate that sphingolipids play a complex, multifactorial role in asthma, yet more remains to be explored

This prediction was experimentally confirmed through Western blot analysis and immunofluorescence assay