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Abbreviations BBD: Box-Behnken design BSA: Bovine serum albumin DTNB: (5,5-dithiobis-(2-nitrobenzoic acid) GPx: Glutathione peroxidase GSH: Reduced glutathione GSSG: Oxidized glutathione LOD: Limits of detection LOQ: Limits of quantitation RSM: Response surface modeling TBuOOH: Tert-Butyl hydroperoxide TNB: Nitro-2-thiobenzoate anion WSAVA: World Small Animal Veterinary Associations References Ademi M (2023) The role of ionized water and its impact on the activity of glutathione peroxidase on rats blood serum during hyperthermic stress

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This paper reports the cryo-electron microscopy structure of the GSDMD pore and reveals its charge-based selectivity for releasing mature IL-1