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This makes it a combinatorial tool of choice for protocols testing synergy with: GIPR agonists (deconstructed tirzepatide, or dedicated GIPR molecules) to explore additive incretin effects FGF21 modulators to study hepatic metabolic synergy SGLT2 inhibitors (empagliflozin, dapagliflozin) to model cardio-renal combinations observed clinically MC4R antagonists to dissect the role of the melanocortin circuit in the central anorexigenic effect Exogenous leptin (in ob/ob) to map leptin-GLP-1 crosstalk in appetite control Synergy studies must plan a 2x2 factorial design (vehicle, compound A alone, compound B alone, combination) to allow statistical interaction analysis
Studies have shown that Chinese medicine affects multiple pathways, including the regulation of nerve growth factors, inflammatory responses, and endocrine axes [232, 529, 530]
References Franceschi, C
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