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One death occurred in a child with autism due to hypocalcemia (low serum calcium levels in the blood) resulting from the inappropriate use of EDTA (a chelator)

Models of dermal fibroblast proliferation, keratinocyte signalling, and wound-closure assays in research environments

Serum limitation-induced senescent fibroblasts model and effect of FOXO4-DRI Passage 3 fibroblasts derived from keloids and normal skins were cultured in a Dulbeccos Modified Eagles medium (DMEM, Hyclone, Logan, Utah, USA) containing 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) to 90% confluence, and then cultured in a DMEM containing 2% FBS for 3 days to construct a serum limitation-induced senescent fibroblast model

Refrigerate upon receipt

Store vials in their original boxes or a dark container within the fridge