A senescence -galactosidase assay was conducted using the senescence -galactosidase staining kit (Cell Signaling Technology, 9860) according to the manufacturers instructions, and then stained cells were imaged using a ZEISS Primovert inverted microscope (Carl Zeiss Microscopy)
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For short-term storage (a few weeks before use), the refrigerator is perfectly fine
Lambert D, Benhayoun S, Adjalla C, Glot MM, Renkes P, Grard P, et al