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of residual GSH in the test tube)/(10 min) * [(Total volume (ml)/ Volume of sample (ml)] * D.F (2) Standard curve To establish a standard curve for the modified GPx-DTNB method, we diluted the glutathione standard with 0.1 M phosphate buffer (pH 7.0) to create a 5 to 500 M concentration range, as shown in Fig