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Agar gels were mixed with 1 mL PBS by vigorous pipetting before the addition of calcein-AM, DCFDA, or mBcl to the same concentration as indicated for cells cultured in liquid medium and incubated for 30 min in the dark at the temperature indicated for those cells
BPC-157 is most commonly used for soft-tissue injury recovery (tendons, ligaments, muscle), chronic tendinopathy, joint discomfort, gut health concerns, and post-surgical tissue repair
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