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In rabies virus G protein, two modalities of neutralization were described: (1) targeting an epitope on a single domain that is fully accessible only in prefusion is enough to lock the protein in prefusion conformation, hence inhibiting the fusion process 44 and (2) recognition of an epitope that spans more than three domains, including one on an adjacent protomer, prevents the conformational changes required for fusion 45

The final benzyl alcohol concentration after dilution into assay media is typically well below the range associated with cellular effects, but vehicle controls remain essential for rigorous experimental design

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10.1080/07388551.2017.1375890 Crit