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If there is one molecule that sits at the intersection of the most important hallmarks of ageing, it is nicotinamide adenine dinucleotide NAD+
-KG is considered an important cofactor for enzymes participating in epigenetic modification 26
Always handle reconstituted solutions using aseptic technique in appropriate laboratory facilities, and document storage conditions as part of standard research protocols
Reduced glutathione (GSH) and glutathione disulfide (GSSG) were quantified by capillary (75-m 50-cm silica) electrophoresis (75 mM boric acid and 25 mM Bis-Tris, pH 8.4, 28C, 18 kV) as described previously (Lavoie et al., 2008)
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