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After blocking, the membrane was incubated overnight at 4 C with the specified primary antibodies, including TRPM2 antibody at a dilution of 1:1000 and GAPDH antibody at a dilution of 1:3000

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Appetite, fullness, and cravings may change earlier for some participants, but early sensations do not reliably predict long-term response or safety

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Porter, Carnitine Metabolism and Human Nutrition, CRC Press, Avr