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1561 proteins (ALS and HC groups) were identified at a 1% false discovery rate, and 699 proteins were quantified in 70% of samples (Additional file 1: Table S2, Additional file 2: Table S3)

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Hold the skin tight at the injection site and insert the needle at a 90-degree angle

A multicellular tumor spheroid is a tissue culture model that mimics the pathological conditions of solid tumors including a three-dimensional cellular structure, nutrition, and proliferation gradients

Masrori et al., 2025)